Environmental Laboratory

  • Fully Automated Mycotoxin Detection in the ppq-Range

Fully Automated Mycotoxin Detection in the ppq-Range

Jan 17 2017 Read 905 Times

LCTech’s robotic system FREESTYLE ThermELUTE enables with its fully automated sample preparation and direct connection to a HPLC system a sample throughput of more than 500 samples per week. The process is done without any manual working steps from extract to the ready chromatogram.

Manual sample preparation is tedious, laborious, and cost-intensive. In combination with a HPLC system the robotic system FREESTYLE ThermELUTE enables fully automated sample preparation and analysis of the mycotoxins Aflatoxin B1, B2, G1, G2, M1 as well as Ochratoxin A and Zearalenone in all regulated matrices.

When developing this system LCTech, specialist in the area of automated sample preparation, has followed the approach of miniaturizing the sample preparation process. Consequently the ThermELUTE is processing immunoaffinity columns with a size of only 3.5 cm, which in turn considerably reduces the processing time as well as the amount of sample and solvent.

The clean-up step itself is performed via thermal denaturation. After being washed, the loaded immunoaffinity column is heated whereby the bond between antibody and toxin is broken. The eluate of the immunoaffinity column is directly and quantitatively eluted from the SMART column, as sample loop partial filling, into the injection port of the LC unit. After derivatisation of the aflatoxins B1 and G1 the eluate is analysed. At the same time the FREESTYLE ThermELUTE begins to process the next sample. With this parallelisation of the two steps, sample preparation and analysis, the sample throughput can be increased enormously.

Moreover, the sensitivity is also drastically increased, because losses through evaporation or adsorption effects are excluded, the complete eluate is injected, and the adjustment to the HPLC mobile phase is omitted. Without special measures or further processing steps the user is able to always measure within the lower ppt-range of the European limits (ppq-range for Aflatoxin M1), even for baby food and to process its application in conformity to EU regulations.

Reader comments

Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.

Post a Comment




Digital Edition

International Environmental Technology April 2019

April 2019

In This Edition Business News - New appointment to boost noise and vibration specialists - Restek and LECO collaborate in worldwide supply agreement - Evonik catalyst in the life support sy...

View all digital editions

Events

IFSE

Apr 25 2019 Bangkok, Thailand

64th ISA Analysis Division Symposium

May 05 2019 Galveston, TX, USA

Ozwater 2019

May 07 2019 Melbourne, Australia

ENVEX

May 15 2019 Seoul, South Korea

FlowExpo 2019

May 16 2019 Guangzhou, China

View all events