Automating DNPH Derivatisation for Aldehyde Analysis

Environmental laboratory

Automating DNPH Derivatisation for Aldehyde Analysis

02 Apr, 2012

Published over 14 years ago. See the latest and most current information on Environmental laboratory.

Dan Carrier
1 min read
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Airborne aldehydes and ketones are collected by passing air through a cartridge containing 2,4-dinitrophenylhydrazine (DNPH). The Supelco cartridge is silica based and pre-coated with DNPH. Carbonyl compounds react with the DNPH to form derivatives in the form of hydrazones which are immobilised on the cartridge. Figure 1 shows the derivatistion of aldehydes and ketones using DNPH.  These compounds can be easily eluted from the cartridge with acetonitrile and analysed by HPLC with UV detection.

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